SSV-F6-040

 “Factors and patterns of the cytotoxic effect of plant photosensitizers”

  • Grant Registration Number – SSV-F6-040
  • Grant venue – Tashkent Medical Academy
  • Funding: 236.55 million sums
  • funding organization – the Ministry of Innovative Development of the Republic of Uzbekistan
  • type of grant – fundamental
  • grant implementation period – 2017-2020

 

Grant principle Investigator

Iriskulov Bakhtiyor Uktamovich

Iriskulov Bakhtiyor Uktamovich

  • Title – Professor, Doctor of Medical Sciences
  • Position – Head of the Department of Normal and Pathological Physiology, part-time head of the Interuniversity Research Laboratory of the Tashkent Medical Academy of the Ministry of Health of the Republic of Uzbekistan (MNIL TMA MZ RUz)
  • Place of work – head of the department of normal and pathological physiology of the Tashkent Medical Academy of the Ministry of Health of the Republic of Uzbekistan (MNIL TMA MZ RUz)
  • Email – bakhtiyor.iriskulov@tma.uz
  • Contact phone: + 99871214-59-45

The perspective of photodynamic therapy (PDT), the technology of the 21st century, is so far from being exhausted. The mechanisms of the cytotoxic effect of plant photosensitizers have not been practically studied. Solving crucial problems and expanding the scope of PDT in medicine are possible with the collaborative research by chemists, biophysicists, physiologists, pathophysiologists and physicians.

A synthetic preparation is used as a photosensitizer for a photodynamic therapy device developed at the Tashkent Medical Academy. Studies of  the Institute of Plant Chemistry of the Academy of Sciences of the Republic of Uzbekistan established that plants in Uzbekistan are sources of natural compounds that synthesize singlet oxygen, which triggers a cascade of free radical reactions that destroy cell membrane organelles.

The aim of this project was to reveal the mechanisms of cellular damage in the photosensitized reaction, underlying the photodynamic action of plant photosensitizers, and to identify factors that enhance and weaken this effect.

Implementation of the project will establish the mechanisms of the initial attachment of plant photosensitizers (PPS) to the outer membrane of cells and penetration into the cell. The severity of cell damage according to our hypothesis depends on the degree of organization of the outer membrane of the cells, type of PPS and the rate of penetration into the cells. Consideration of these factors will possibly increase the effectiveness of photodynamic therapy depending on the type of cells and tissues, the degree of blood supply, the type of PPS and the light source.

The project, at the end, will provide fundamental knowledge about the levels of exposure to quantum radiation, contributing to the destruction of the membranes of intracellular structures, the development of apoptosis and the cell death. The decisive factor in the selectivity of exposure is the intensity of cell division when using plants synthesizing singlet oxygen (the trigger mechanism of the cascade of free radical reactions leading to the destruction of cell structures and its death).

Based on the results of the project, a monograph will be published outlining new established scientific doctrines that will determine the possibility of using PDT in the ophthalmology, dentistry, purulent surgery, pathobiology, dermatology, etc.

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